MIA Polyclonal Antibody
Catalogue Numbers: BS61209-50, BS61209-100
Sizes: 50µl, 100µl
Alternative Name: Melanoma-derived growth regulatory protein; Melanoma inhibitory activity protein; MIA
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q16674
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Tumorigenesis is a process that is mediated by a network of membrane, cytosolic and nuclear associated factors, which regulate proliferation and cell-matrix interaction through signaling cascades. The phenotype of malignant melanomas in vivo depends on the global expression of stimulatory or inhibitory factors generated in both the tumors cells and their environment. One example, Melanoma inhibitory activity (cartilage-derived retinoic acid-sensitive protein (CD-RAP), MIA) is a Src homology 3 (SH3)-like domain containing protein that is secreted from chondrocytes and malignant melanoma cells. MIA is translated as a 131-amino acid pro-form and processed into a mature 107-amino acid protein after cleavage of a secretion signal. MIA is expressed during chondrogenesis and in mature chondrocytes, suggesting that MIA is necessary for normal cartilage cell phenotype. MIA mRNA is present in carcinomas of the colon, ovary, kidney, and head/neck, and may represent a marker to monitor melanomic activity.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MIA polyclonal antibody detects endogenous levels of MIA protein.
Bioworld Molecular Weight: ~ 14 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of MIA pAb at 1:500 dilution
Lane 1: BV2 whole cell lysate(40ug)
Lane 2: C6 whole cell lysate(40ug)
Lane 3: K562 whole cell lysate(40ug)
Lane 4: A2780 whole cell lysate(40ug)
Lane 5: U-87MG whole cell lysate(40ug)
Lane 6: HEK293T whole cell lysate(40ug)
Immunogen: A synthetic peptide corresponding to residues in Human MIA.
Conjugate: Unconjugated
Modification: Unmodified