Product Description

MIF Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90859-50, BS90859-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14174(Human) P34884(Mouse) P30904(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP, , FC

All Applications: WB:1:500-1:2,000
IP:1:10-1:50
FC:1:50-1:100

Background: Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor α (TNFα), interleukin-1 (IL-1) and interferon-γ (IFN-γ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MIF polyclonal antibody detects endogenous levels of MIF protein.

Bioworld Molecular Weight: 12 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MIF on different cells lysates using anti-MIF antibody at 1/500 dilution.
Positive control:
Line 1: Mouse brain
Line 2: Jurkat
Line 3: Hela, Flow cytometric analysis of THP-1 cells with MIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: MIF, GIF, GIF, GLIF, Glycosylation inhibiting factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Macrophage migration inhibitory factor (glycosylation-inhibiting factor), Macrophage migration inhibitory factor, MIF, MIF protein, MIF, MMIF, Phenylpyruvate tautomerase,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified