Product Description

Mindin (W104) Polyclonal Antibody

Catalogue Numbers: BS9256-50, BS9256-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BUD6

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Thrombospondin proteins, Thrombospondins 1-4 and Thrombospondin 5 (also designated COMP), compose a family of glycoproteins that are involved in cell-to-cell and cell-to-matrix signaling. These extracellular, cell-surface proteins form complexes of both homo- and heteromultimers. Spondin-2, or Mindin, is also designated DIL-1 for its differential expression in cancerous and non-cancerous lung cells. Full-length SPON2 cDNA encodes a 331 amino acid protein with a domain arrangement similar to zebrafish F-Spondin and Mindin-1/Mindin-2: an FS1 domain, an FS2 domain, a hydrophobic signal sequence in the N-terminus and a Thrombospondin type I repeat. Immunoblot analysis demonstrates expression of a 42 kDa protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Mindin (W104) polyclonal antibody detects endogenous levels of Mindin protein.

Extra Notes: Western blot (WB) analysis of SPON2 pAb at 1:2000 dilution
Lane1:PC12 whole cell lysate(40ug)
Lane2:LOVO whole cell lysate(40ug)
Lane3:SGC7901 whole cell lysate(40ug)
Lane4:HEK293T whole cell lysate(30ug)

Bioworld Molecular Weight: ~ 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DIL 1; DIL-1; DIL1; M spondin; Mindin; SPON 2; Spon2; Spondin 2; Spondin-2;

Immunogen: Synthetic peptide, corresponding to amino acids 70-118 of Human Mindin.

Conjugate: Unconjugated

Modification: Unmodified