Mini Samples ELISA Kit for Angiotensin II (AngII)
Sizes: 24T, 48T, 96T
Catalogue Numbers: MEA005Mu-24, MEA005Mu-48, MEA005Mu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Mini samples kit
Target: Angiotensin II
Alternative Names: Ang-II; Angiotensin-2
Target Species: Mouse
Uniprot: P11859
Featured Series Function: Detects protein (micro-volume samples 15-25 μl)
Specificity: Reactive with Mouse AngII / Angiotensin II
Method: Colormetric
Detection principle: Competitive Inhibition
Detection
range: 3.70-300pg/mL
Sensitivity: 1.27pg/mL
Assay Time: 3h
Sample Size: 15-25uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Angiotensin II (AngII).
No significant cross-reactivity or interference between Mini Samples Angiotensin II (AngII) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
And then add 25μL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to AngII has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled AngII and unlabeled AngII (Standards or samples) with the pre-coated antibody specific to AngII. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of AngII in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of AngII in the sample.
Research Area: Endocrinology; Cardiovascular biology;
Research Use Only