Mini Samples ELISA Kit for Collagen Type I (COL1)
Sizes: 24T, 48T, 96T
Catalogue Numbers: MEA571Hu-24, MEA571Hu-48, MEA571Hu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Mini samples kit
Target: Collagen Type I
Target Species: Human
Uniprot: P02452
Gene ID: 1277
Featured Series Function: Detects protein (micro-volume samples 15-25 μl)
Specificity: Reactive with Human COL1 / Collagen Type I
Method: Colormetric
Detection principle: Double-antibody Sandwich
Detection
range: 0.156-10ng/mL
Sensitivity: 0.054ng/mL
Assay Time: 3h
Sample Size: 15-25uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Collagen Type I (COL1).
No significant cross-reactivity or interference between Mini Samples Collagen Type I (COL1) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Collagen Type I (COL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Collagen Type I (COL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Collagen Type I (COL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Collagen Type I (COL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Collagen Type I (COL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Collagen Type I (COL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Metabolic pathway; Developmental science; Bone metabolism;
Research Use Only