Mini Samples ELISA Kit for Interleukin 35 (IL35)
Sizes: 24T, 48T, 96T
Catalogue Numbers: MEC008Mu-24, MEC008Mu-48, MEC008Mu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Mini samples kit
Target: Interleukin 35
Target Species: Mouse
Uniprot: P43431
Gene ID: 16159
Featured Series Function: Detects protein (micro-volume samples 15-25 μl)
Specificity: Reactive with Mouse IL35 / Interleukin 35
Method: Colormetric
Detection principle: Double-antibody Sandwich
Detection
range: 7.8-500pg/mL
Sensitivity: 3.1pg/mL
Assay Time: 3h
Sample Size: 15-25uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 35 (IL35).
No significant cross-reactivity or interference between Mini Samples Interleukin 35 (IL35) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 35 (IL35) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 35 (IL35) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to IL12A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated monoclonal antibody specific to IL27B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL35 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Cytokine; Tumor immunity; Infection immunity;
Research Use Only