Mini Samples ELISA Kit for Pentraxin 3, Long (PTX3)
Sizes: 24T, 48T, 96T
Catalogue Numbers: MEK411Hu-24, MEK411Hu-48, MEK411Hu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Mini samples kit
Target: Pentraxin 3, Long
Alternative Names: TNFAIP5; TSG-14; Pentaxin-Related Gene, Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein
Target Species: Human
Uniprot: P26022
Gene ID: 5806
Featured Series Function: Detects protein (micro-volume samples 15-25 μl)
Specificity: Reactive with Human PTX3 / Pentraxin 3, Long
Method: Colormetric
Detection principle: Double-antibody Sandwich
Detection
range: 93.75-6,000pg/mL
Sensitivity: 34.51pg/mL
Assay Time: 3h
Sample Size: 15-25uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Pentraxin 3, Long (PTX3).
No significant cross-reactivity or interference between Mini Samples Pentraxin 3, Long (PTX3) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Pentraxin 3, Long (PTX3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Pentraxin 3, Long (PTX3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Pentraxin 3, Long (PTX3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Pentraxin 3, Long (PTX3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Pentraxin 3, Long (PTX3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Pentraxin 3, Long (PTX3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Tumor immunity;
Research Use Only