MIS12 Polyclonal Antibody-BS76345

MIS12 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76345-50, BS76345-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H081

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:200 - 1:2000

Background: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using MIS12 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 24kDa

Alternative Name: MIS12; 2510025F08Rik; KNTC2AP; MTW1; hMis12

Immunogen: Recombinant fusion protein of human MIS12(NP_076944.1).

Conjugate: Unconjugated

Modification: Unmodified