Product Description

MITF (phospho-S180) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS4328-50, BS4328-100

Sizes: 50µl, 100µl

Swiss-Prot: O75030

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

Background: MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-MITF (S180) polyclonal antibody detects endogenous levels of MITF protein only when phosphorylated at Ser180

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of p-MITF (S180) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 59 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32; MITF; BHLHE32

Immunogen: Synthetic phosphopeptide derived from human MITF around the phosphorylation site of Serine 180.

Conjugate: Unconjugated

Modification: Phosphorylation