Product Description

Mitofusin 1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90863-50, BS90863-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8IWA4(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, IP, , FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: Mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) are homologs for the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins and are mediators of mitochondrial fusion. A GTPase domain is required for Mfn protein function but the molecular mechanisms of the GTPase-dependent reaction as well as the functional division of the two Mfn proteins are unknown. They are essential for embryonic development and may play a role in the pathobiology of obesity. Although the Mfn1 and Mfn2 genes are broadly expressed, they show different levels of expression in different tissues. Two Mfn1 transcripts are elevated in heart, while Mfn2 mRNA is abundantly expressed in heart and muscle tissue but present only at low levels in many other tissues. Mfn1 localizes to mitochondria and participates in at least two different high molecular weight protein complexes in a GTP-dependent manner. Purified recombinant Mfn1 exhibited approximately eightfold higher GTPase activity than Mfn2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Mitofusin 1 polyclonal antibody detects endogenous levels of Mitofusin 1 protein.

Bioworld Molecular Weight: 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Mitofusin 1 on K562 cells lysates using anti-Mitofusin 1 antibody at 1/1,000 dilution., Flow cytometric analysis of 293 cells with Mitofusin 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: MFN1, Fzo homolog, Hfzo1, Hfzo2, MFN 1, Mfn1, MFN1, Mitochondrial transmembrane GTPase Fzo 1, Mitochondrial transmembrane GTPase FZO 2, Mitochondrial transmembrane GTPase FZO1B, Mitofusin 1, Mitofusin-1, Mitofusin1, MS996, Putative transmembrane GTPase, Transmembrane GTPase MFN1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified