Product Description

MKK5 (Phospho-S311) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64556-50, BS64556-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13163

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MKK5 (Phospho-S311) polyclonal antibody detects endogenous levels of MKK5 protein only when phosphorylated at Ser311.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of MKK5 (Phospho-S311) polyclonal antibody at 1:500 dilution
Lane A: The Heart tissue lysate of Mouse
Lane B: The Heart tissue lysate of Rat
Lane C: H1792 whole cell lysate
Lane D: MCF-7 whole cell lysate

Alternative Name: Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAPKK 5; MAPK/ERK kinase 5; MEK 5; MAP2K5; MEK5, MKK5, PRKMK5

Immunogen: Synthetic phosphopeptide derived from human MKK5 around the phosphorylation site of Serine 311.

Conjugate: Unconjugated

Modification: Phosphorylation