MLCK (Phospho Tyr471) rabbit pAb - ES20185

MLCK (Phospho Tyr471) rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES20185-50, ES20185-100

Citations, Manuals and MSDS Available upon request.

Background: myosin light chain kinase (MYLK) Homo sapiens This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been

Alternate Name: Myosin light chain kinase, smooth muscle (MLCK; smMLCK; EC 2.7.11.18; Kinase-related protein; KRP; Telokin) [Cleaved into: Myosin light chain kinase, smooth muscle, deglutamylated form]

Source: Rabbit

Applications: WB; IHC

Dilution: WB 1:500-2000; IHC-p 1:50-300

Reactivity: Human; Mouse; Rat

Immunogen: Synthesized peptide derived from human MLCK (Phospho Tyr471)

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 200kD

Human Gene ID: 4638

Human SWISS Prot NO: Q15746

Subcellular Location: Cytoplasm. Cell projection, lamellipodium. Cleavage furrow. Cytoplasm, cytoskeleton, stress fiber. Localized to stress fibers during interphase and to the cleavage furrow during mitosis.

Research Use Only