Product Description

MLF1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5797-50, BS5797-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Myeloid leukemia factor 1 (MLF1) is a 268 amino acid protein expressed by a gene that is involved in translocations associated with acute myeloid leukemia. MLF1 is a widely expressed negative regμlator of cell cycle progression functioning upstream of the tumor suppressor p53. MLF1 induces p53-dependent cell cycle arrest in murine embryonic fibroblasts. MLF1 expression also inversely affects the endogenous level of COP1, a ubiquitin ligase for p53, inhibits Epo-induced cell cycle exit, and inhibits a rise in the cell cycle inhibitor p27. Polo-like kinase 1 (Plk1) phosphorylates MLF1 at its Thr78 site, which induces ubiquitination and degradation of MLF1 before the transition from metaphase to anaphase. Mutations of these phosphorylation sites stabilize MLF1 and inhibit mitotic progression. MLF1 normally functions in mμlti-potent progenitor cells, and its dysregμlation may be somewhat responsible for leukemogenesis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF-1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 194-239 of Human MLF1.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MLF1 polyclonal antibody detects endogenous levels of MLF1 protein.

Molecular Weight: ~ 31 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P58340

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only