MLH1 Rabbit Monoclonal Antibody-MB66267

MLH1 Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66267-50, MB66267-100

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: P40692

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Background: Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α), or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases, and DNA ligase. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). MLH1 also plays a role in meiotic recombination.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of MLH1 protein.

Bioworld Molecular Weight: ~ 83 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MLH1 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates., Immunofluorescent analysis of MLH1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1

Immunogen: A synthetic peptide of human MLH1

Conjugate: Unconjugated

Modification: Unmodified