Product Description

MLH1 (S467) Polyclonal Antibody

Catalogue Numbers: BS2418-50, BS2418-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P40692

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: MLH1 is an integral part of the protein complex responsible for mismatch repair that is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MLH1 (S467) polyclonal antibody detects endogenous levels of MLH1 protein.

Extra Notes: Western blot (WB) analysis of MLH1 (S467) pAb at 1:500 dilution
Lane1:PC12 whole cell lysate(40ug)
Lane2:BV2 whole cell lysate(40ug)
Lane3:PC3 whole cell lysate(40ug)
Lane4:A549 whole cell lysate(40ug)
Lane5:MCF-7 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2

Immunogen: Synthetic peptide, corresponding to amino acids 450-500 of Human MLH1.

Conjugate: Unconjugated

Modification: Unmodified