Product Description

MLK3 Polyclonal Antibody

Catalogue Numbers: BS64014N-50, BS64014N-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q16584

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Mixed lineage kinase 3 (MLK3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecular phosphorylation and subsequent activation of MLK3. Autophosphorylation of Thr277 and Ser281 is essential for MLK3 kinase activity. Ser281 is also phosphorylated by HPK in an in vitro kinase assay. MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IκB kinase and mediates the activation of the transcriptional factor NF-κB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MLK3 polyclonal antibody detects endogenous levels of MLK3 protein.

Extra Notes: Western blot (WB) analysis of MLK3 polyclonal antibody at 1:1000 dilution
Lane1:HEK293T whole cell lysate(30ug)
Lane2:NIH-3T3 whole cell lysate(30ug)

Bioworld Molecular Weight: ~ 92 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase; MAP3K11; MLK3; PTK1; SPRK

Immunogen: Synthetic peptide, corresponding to Human MLK3.

Conjugate: Unconjugated

Modification: Unmodified