Product Description

MMADHC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76489-50, BS76489-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H3L0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin. Pseudogenes have been identified on chromosomes 11 and X.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using MMADHC antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 33kDa

Alternative Name: MMADHC; C2orf25; CL25022; cblD; cblD type

Immunogen: Recombinant fusion protein of human MMADHC(NP_056517.1).

Conjugate: Unconjugated

Modification: Unmodified