MMP-1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90866-50, BS90866-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P03956
Host: Rabbit
Reactivity: Human
Applications: WB, ICC IHC
All Applications: WB:1:1,000
ICC:1:200
IHC:1:200
Background: MMP-1 has an archetypal structure consisting of a pre-domain, a pro-domain, a catalytic domain, a linker region and a hemopexin-like domain. Two main nomenclatures for the primary structure are currently in use, the original one from which the first amino-acid starts with the signalling peptide and a second one where the first amino-acid starts counting from the prodomain (proenzyme nomenclature). MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Specifically, MMP-1 breaks down the interstitial collagens, types I, II, and III. Mechanical force may increase the expression of MMP1 in human periodontal ligament cells.
Purification and Purity: Peptide affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MMP-1 polyclonal antibody detects endogenous levels of MMP-1 protein.
Bioworld Molecular Weight: 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.
Positive control:
Lane 1: SKBR3
Lane 2: Raji
Lane 3: A431
Lane 4: A549, ICC staining MMP1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MMP-1, 27 kDa interstitial collagenase, CLG, CLGN, collagenase, fibroblast, collagenase, interstitial, Fibroblast collagenase, Interstitial collagenase, Matrix metallopeptidase 1 (interstitial collagenase), Matrix metalloprotease 1, Matrix Metalloproteinase 1, Matrix metalloproteinase-1, MMP 1, MMP-1, MMP1, MMP1, OTTHUMP00000045866,
Immunogen: peptide
Conjugate: Unconjugated
Modification: Unmodified