Product Description

MMP-12 (D411) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS3699-50, BS3699-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Matrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insμlin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3), it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimμlated macrophages produce MMP-12, it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimμlation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Macrophage metalloelastase, MME, 3.4.24.65, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human MMP-12.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MMP-12 (D411) polyclonal antibody detects endogenous levels of MMP-12 protein.

Molecular Weight: ~ 48 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P39900

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only