MMP-13 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90868-50, BS90868-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P45452(Human) P33435(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen types I, II and III. MMP-13 has wide substrate specificity, and its physiologic expression is limited to situations in which rapid and effective remodeling of collagenous ECM takes place, such as fetal bone development and adult bone remodeling.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: MMP-13 polyclonal antibody detects endogenous levels of MMP-13 protein.
Bioworld Molecular Weight: 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of MMP13 on MCF-7 cells lysates using anti-MMP13 antibody at 1/1,000 dilution., ICC staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MMP-13, MMP13, CLG 3, CLG3, Collagenase 3, Collagenase3, MANDP1, Matrix metallopeptidase 13 (collagenase 3), Matrix Metalloproteinase 13, Matrix metalloproteinase-13, MMP 13, MMP-13, Mmp13, MMP13,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified