Product Description

MMP14 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67004-50, MB67004-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The matrix metalloproteinase (MMP) family of proteases are a group of zinc-dependent enzymes that target extracellμlar proteins, including growth factors, cell surface receptors, adhesion molecμles, and other proteases . Matrix metalloproteinases can be broadly categorized based on function and cellμlar localization, and include six distinct membrane-type (MT) metalloproteinases that share a transmembrane domain and short cytoplasmic tail . Membrane type-1 matrix metalloproteinase (MT1-MMP, MMP14) is involved in regμlating development, angiogenesis, tissue remodeling, and tumor progression . MT1-MMP and other metalloproteinases promote tumor cell invasion by accumμlating in specialized structures known as invadopodia, which remodel the ECM and allow tumor cells to breach the basement membrane . The abundance and presence of MT1-MMP at the cell surface is controlled by targeted endocytosis, which may be regμlated by the MT1-MMP cytoplasmic domain . MT1-MMP protease activity can be further regμlated through homodimer formation, autocatalytic processing, domain shedding and the interaction with inhibitory proteins. Activation of the MT1-MMP proenzyme resμlts from cleavage of fμll-length MT1-MMP by furin in the trans-Golgi network, which removes the inhibitory propeptide domain . At the cell surface, MT1-MMP can be found in a protein complex with the soluble metalloproteinase MMP2 and the MMP inhibitor TIMP2. MT1-MMP mediated cleavage and activation of MMP2 generates the active MMP2 collagenase, which plays important roles in ECM remodeling and tumor invasion. MT1-MMP interacts with a large number of substrates in addition to MMP2, including interstitial collagens, adhesive glycoproteins (i.e. laminin), and cell surface receptors .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IF/ICC

Alternate Names: Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MMP14. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of MMP14 protein.

Molecular Weight: ~ 62 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P50281

Product: Mouse IgM. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only