MMP20 Polyclonal Antibody
Catalogue Numbers: BS60659-50, BS60659-100
Sizes: 50µl, 100µl
Alternative Name: Matrix metalloproteinase-20; MMP-20; Enamel metalloproteinase; Enamelysin; MMP20
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O60882
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Matrix metalloproteinases (MMPs) are highly homologous Zn2+ endopeptidases involved in extracellular matrix (ECM) breakdown. MMP-20 (enamelysin) is involved in the degradation of various components of the ECM during development, hemostasis and pathological conditions. The domain organization of MMP-20 is similar to other MMPs, including a signal peptide, a prodomain with the conserved motif PRCGVPD involved in maintaining enzyme latency, a catalytic domain with a Zn-binding site, and a COOH-terminal fragment similar to the sequence of hemopexin. MMP-20 is expressed during the early through middle stages of enamel development at which time it likely hydrolyzes Amelogenin, a major protein component of the enamel matrix. The expression pattern of MMP-20 in the enamel organ indicates that it may be involved in the turnover of ECM proteins during tooth development and enamel formation. Human MMP-20 maps to chromosome 11q22.3, clustered to at least seven other members of the MMP gene family.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: MMP20 polyclonal antibody detects endogenous levels of MMP20 protein.
Bioworld Molecular Weight: ~ 54 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of MMP20 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human MMP20
Conjugate: Unconjugated
Modification: Unmodified