Product Description

MOBP Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65670-50, BS65670-100

Swiss-Prot: Q13875

Host: Rabbit

MOBP Polyclonal Antibody-BS65670

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: The gene encoding MOBP a member of the central nervous system myelin-constituting proteins, maps to chromosome 3p22.2. MOBP has many splice variants that share a 68 amino acid N-terminal domain. MOBP-71, MOBP-81A, MOBP-99, and MOBP-169 are MOBP splice variants that contain exon 8b, which is similar to myelin basic protein (MBP) mRNA RTS, however MOBP-69, MOBP-81B, and MOBP-170 lack this exon. The splice variants that contain exon 8b are expressed in myelin, while those lacking exon 8b are retained in the oligodendrocyte soma. Exon 8b-containing variants are directed to sites of myelin sheath assembly by exon 8b, where they play a structural role in myelin formation. Splice variants lacking exon 8b likely play a cellular and/or regulatory role. MOBP is implicated in multiple sclerosis (MS), a human demyelinating disease, and in allergic encephalomyelitis in rodents.

Purification and Purity: affinity purified by Protein A

Specificity: MOBP Polyclonal Antibody detects endogenous levels of MOBP protein.

Bioworld Molecular Weight: 21kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Positive sample：Paraformaldehyde-fixed, paraffin embedded (mouse brian)Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat liver)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Positive sample：Paraformaldehyde-fixed, paraffin embedded (rat brian)Negative sample：Paraformaldehyde-fixed, paraffin embedded (rat liver)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOBP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: MGC87379; Myelin associated oligodendrocyte basic protein; MOBP_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human MOBP

Modification: Unmodified

Conjugate: Unconjugated