Product Description

MonoMethyl-Histone H3-R17 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS8451-50, BS8451-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecμles of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Other (Wide Range)

Host: Rabbit

Applications: WB, IHC, IF/ICC, IP, ChIP, ChIP-seq

Alternate Names: HIST1H3J, H3/j, H3FJ

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Methylated

Immunogen: A synthetic monomethylated peptide around R17 of human histone H3(NP_003520.1).

Dilution: WB, 1:500 - 1:2000 IHC, 1:50 - 1:200 IF/ICC, 1:50 - 1:200 IP, 1:50 - 1:200 ChIP, 1:20 - 1:100 ChIP-seq, 1:20 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Methylated Antibodies

Molecular Weight: 17kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P68431

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only