MonoMethyl-UHRF1-K385 Polyclonal Antibody-BS74109

MonoMethyl-UHRF1-K385 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74109-50, BS74109-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96T88

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: DB, WB, IHC

All Applications: DB,1:500 - 1:2000 | WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Methylated

Bioworld Molecular Weight: 91kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of C6 cells, using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.C6 cells were treated by MG132 at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s., Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-UHRF1-K385 antibody at 1:1000 dilution.

Alternative Name: UHRF1; ICBP90; Np95; RNF106; TDRD22; hNP95; hUHRF1; huNp95

Immunogen: A synthetic monomethylated peptide around K385 of human UHRF1(NP_001041666.1).

Conjugate: Unconjugated

Modification: Methylated