Mouse APLN (Apelin) ELISA Kit - ELK7012

Mouse APLN (Apelin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7012-48, ELK7012-96, ELK7012-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: XNPEP2; APEL; APJ endogenous ligand

Assay Type: Competitive Inhibition

Sensitivity: 34.8 pg/mL

Standard: 8000 pg/mL

Detection Range: 125-8000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9R0R4

Research Use Only