Mouse DPP8 (Dipeptidyl Peptidase 8) ELISA Kit - ELK6504

Mouse DPP8 (Dipeptidyl Peptidase 8) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6504-48, ELK6504-96, ELK6504-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: DP8; DPRP1; MSTP141; MSTP097; MSTP135; dipeptidylpeptidase 8; Dipeptidyl Peptidase IV-Related Protein-1; Prolyl Dipeptidase DPP VIII

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DPP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DPP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DPP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DPP8 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q80YA7

Research Use Only