Mouse FUT8 (Fucosyltransferase 8) ELISA Kit - ELK7032

Mouse FUT8 (Fucosyltransferase 8) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7032-48, ELK7032-96, ELK7032-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; Alpha- (1,6)-fucosyltransferase

Assay Type: Sandwich

Sensitivity: 0.124 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FUT8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FUT8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FUT8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FUT8 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9WTS2

Research Use Only