Mouse GREM1 (Gremlin 1) ELISA Kit - ELK6358

Mouse GREM1 (Gremlin 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6358-48, ELK6358-96, ELK6358-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

Assay Type: Sandwich

Sensitivity: 0.059 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Tumor immunity; Developmental science; Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GREM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O70326

Research Use Only