Mouse IgA (Immunoglobulin A) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-OSEL-M0007-24, E-OSEL-M0007-48, E-OSEL-M0007-96
Citations, Manuals and MSDS Available upon request.
Abbreviation: IgA
UNIProt ID: ???
Target Synonyms: Immunoglobulin A; IgA;
Research Areas: Cancer, Immunology
Target Species: Mouse
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse IgA concentrations in serum, plasma.
Tested Sample Types: Serum, Plasma
Type: Sandwich
Assay Time: 1.5h
Detection Type: Colormetric
Sensitivity: 0.94 ng/mL
Detection Range: 1.56-100 ng/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgA are added to the micro ELISA plate wells. Mouse IgA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgA.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IgA. The concentration of Mouse IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Mouse IgA in samples. No significant cross-reactivity or interference between Mouse IgA and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃, 6 months
Shipping: ice bag