Mouse ISR (Insulin Receptor) ELISA Kit - ELK4976

Mouse ISR (Insulin Receptor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4976-48, ELK4976-96, ELK4976-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CD220; INSR; HHF5; IR; Insulin receptor subunit beta; Insulin Receptor beta

Assay Type: Sandwich

Sensitivity: 11.8 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Endocrinology; Autoimmunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ISR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ISR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ISR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ISR in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P15208

Research Use Only