Mouse Monoclonal Antibody to HCV Subtype 1B NS5B Protein - A2-424-100

Mouse Monoclonal Antibody to HCV Subtype 1B NS5B Protein

Size: 100 µg

Catalogue Number: A2-424-100

Citations, Manuals and MSDS Available upon request.

Immunogen: HCV 1b NS5B

Immunogen Description: Recombinant Hepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Background: Non-structural protein 5B (NS5B) represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, which is a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronic hepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunit RNA-dependent RNA polymerase is absolutely essential for the viral replication.

UniProt ID: Q9WMX2

Clonality: Mouse monoclonal

Clone: 9A2

Class: IgG1

Reactivity: Human, HCV subtype 1b NS5B, Epitope mapped to amino acids 111-130 (LSSKAVNHIRSVWKDLLEDT)

Application: ELISA, WB, IP, IF

Protocol: WB in reduced conditions 1 µg/ml; IF 2 µg/ml
Optimal conditions for IP should be determined for each particular application.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Purification: Protein G purification

Buffer: PBS pH 7.4, with 0.1% sodium azide

Shipping: This product is shipped in non-frozen liquid form in ambient conditions

Storage: Store at - 20 to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.

Research Use Only