Mouse Monoclonal Antibody to Human Cardiac Troponin I
Size: 100 µg
Catalogue Number: 105-100
Citations, Manuals and MSDS Available upon request.
Immunogen: Human cardiac troponin I
Immunogen Description: Native, affinity purified human cardiac troponin I
Background: Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle (Wikipedia)
UniProt ID: P19429
Clonality: Mouse monoclonal
Clone: 5C5
Class: IgG1
Reactivity: Human cardiac troponin I
Dissociation constant (KD): 1.9x 10-9 M
Application: ELISA, WB
Protocol: ELISA, 0.05-0.1 μg/ml; WB, 0.2 to 0.5 μg/ml
Purification: Protein G purification
Buffer: PBS pH 7.4; 0.1% NaN3
Shipping: This product is shipped in non-frozen liquid form in ambient conditions
Storage: Store at –20 to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Research Use Only