Mouse TGFb2 (Transforming Growth Factor Beta 2) ELISA Kit - ELK5216

Mouse TGFb2 (Transforming Growth Factor Beta 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5216-48, ELK5216-96, ELK5216-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor

Assay Type: Competitive Inhibition

Sensitivity: 8.95 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Cytokine; Infection immunity;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P27090

Research Use Only