Mouse TUBa4A (Tubulin Alpha 4A) ELISA Kit - ELK6809

Mouse TUBa4A (Tubulin Alpha 4A) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6809-48, ELK6809-96, ELK6809-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TUBA1; Testis Specific Tubulin Alpha 1; Alpha-tubulin 1; Tubulin alpha-1 chain; Tubulin H2-alpha

Assay Type: Sandwich

Sensitivity: 0.127 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Neuroscience

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TUBa4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TUBa4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TUBa4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TUBa4A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P68368

Research Use Only