MPG Polyclonal Antibody-BS60143

MPG Polyclonal Antibody

Catalogue Numbers: BS60143-50, BS60143-100

Sizes: 50µl, 100µl

Alternative Name: DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase; AAG; ANPG; MID1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29372

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Maintenance of DNA sequences is necessary for vertebrates and other life. DNA is under constant stress by a plethora of DNA-damaging agents present in both the environment and within cells. The potentially deleterious effects of DNA lesions in cells are elegantly resolved by sophisticated DNA repair systems, including base excision repair (BER), nucleotide excision repair (NER) and DNA repair methyltransferase (MTase). Methylated bases, such as 3-methyladenine (3MeA) and 7-methylguanine (7MeG) can be formed by agents in the environment and by endogenous cellular processes. Consequently, in the absence of exposure to environmental agents, DNA methylation damage can be incurred on the genomic DNA of normal mammalian cells. DNA N-glycosylases are base excision-repair proteins that locate and cleave damaged bases from DNA as the first step in restoring the sequence. 3MeA DNA glycosylases initiate base excision repair by removing 3MeA. These glycosylases also remove a broad spectrum of spontaneous and environmentally induced base lesions. The human N-methylpurine-DNA glycosylase gene maps to chromosome 16p13.3 and encodes a 298 amino acid protein, known as APNG.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MPG polyclonal antibody detects endogenous levels of MPG protein.

Bioworld Molecular Weight: ~ 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MPG polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human MPG.

Conjugate: Unconjugated

Modification: Unmodified