MPP10 Polyclonal Antibody-BS5799

MPP10 Polyclonal Antibody

Catalogue Numbers: BS5799-50, BS5799-100

Sizes: 50µl, 100µl

Alternative Name: U3 small nucleolar ribonucleoprotein protein MPP10; M phase phosphoprotein 10; MPHOSPH10

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00566

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: MPP10 (M-phase phosphoprotein 10), also known as MPHOSPH10, is a 681 amino acid protein that localizes to the fibrillar region of the nucleolus. Phosphorylated in the M (mitotic) phase of the cell cycle, MPP10 is a component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex and is required for the early cleavages during pre-18S ribosomal RNA processing. MPP10 forms a heterotrimeric complex with IMP-3 and IMP-4, which may be required for its association with nucleolar components. The gene encoding MPP10 is localized to human chromosome 2, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MPP10 polyclonal antibody detects endogenous levels of MPP10 protein.

Bioworld Molecular Weight: ~ 79 kDa, 120 kDa (Phosphorylated)

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MPP10 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 126-171 of Human MPP10.

Conjugate: Unconjugated

Modification: Unmodified