MRCKβ (F1665) Polyclonal Antibody-BS2382

MRCKβ (F1665) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2382-50, BS2382-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9Y5S2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Myotonic dystrophy kinaserelated Cdc42-binding (DMPK-like) kinases-α and β (MRCK-α, β) contain a cysteine-rich motif and a putative pleckstrin homology domain. MRCKs can phosphorylate nonmuscle Myosin light chain and influences Actin-Myosin contractility. MRCK-α can phosphorylate and activate LIM kinases downstream of Cdc42, which leads to inactivation of ADF/Cofilin and to Actin cytoskeletal reorganization. MRCK-α can also influence neurite outgrowth promoted by Cdc42 and Rac.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MRCKβ (F1665) polyclonal antibody detects endogenous levels of MRCKβ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRCKβ (F1665) pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Mouse (40ug)
Lane 2: The Brain tissue lysate of Rat (40ug)
Lane 3: A549 whole cell lysate (40ug)
Lane 4: MCF-7 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of MRCKβ (F1665) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 194 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Serine/threonine-protein kinase MRCK beta; CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta;Myotonic dystrophy protein kinase-like beta; CDC42BPB; KIAA1124

Immunogen: Synthetic peptide, corresponding to amino acids 1630-1680 of Human MRCKβ.

Conjugate: Unconjugated

Modification: Unmodified