MRE11 (L260) Polyclonal Antibody-BS1852

MRE11 (L260) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1852-50, BS1852-100

Sizes: 50µl, 100µl

Swiss-Prot: P49959

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination..

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: MRE11 (L260) polyclonal antibody detects endogenous levels of MRE11 protein

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRE11 (L260) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (2.5ug)
Lane 2: K562 whole cell lysate (2.5ug)
Lane 3: The Brain tissue lysate of Rat (40ug)
Lane 4: The Brain tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of MRE11 (L260) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 81 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A; MRE11A; HNGS1; MRE11

Immunogen: Synthetic peptide, corresponding to amino acids 240-280 of Human MRE11.

Conjugate: Unconjugated

Modification: Unmodified