Product Description

MRP-S12 Polyclonal Antibody

Catalogue Numbers: BS5803-50, BS5803-100

Sizes: 50µl, 100µl

Alternative Name: 28S ribosomal protein S12, mitochondrial; MRP-S12; S12mt; MT-RPS12; MRPS12; RPMS12; RPSM12

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15235

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Mitochondrial ribosomes consist of a large 39S subunit and a small 28S subunit, both of which are comprised of multiple mitochondrial ribosomal proteins (MRPs) that are encoded by nuclear genes and are essential for protein synthesis within mitochondria. MRP-S12 (mitochondrial ribosomal protein S12) is a 138 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. In response to mitochondrial stress, bidirectional MRP-S12 promoter activity is strongly stimulated, an event that happens to correlate with mitochondrial reactive oxidative species (ROS) production. Due to its specific location on human chromosome 19, the gene encoding MRP-S12 may be a candidate gene for susceptibility to aminoglycoside ototoxicity and for the autosomal dominant deafness gene DFNA4.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP-S12 polyclonal antibody detects endogenous levels of MRP-S12 protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRP-S12 (K59) pAb at 1:1000 dilution
Lane 1: AML-12 whole cell lysate(40ug)
Lane 2: H9C2 whole cell lysate(40ug)
Lane 3: HCT116 whole cell lysate(40ug)
Lane 4: L02 whole cell lysate(40ug)
Lane 5: Hela whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 69-114 of Human MRP-S12.

Conjugate: Unconjugated

Modification: Unmodified