Product Description

MRP-S35 (I252) Polyclonal Antibody

Catalogue Numbers: BS3782-50, BS3782-100

Sizes: 50µl, 100µl

Alternative Name: 28S ribosomal protein S35, mitochondrial; MRP-S35; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt; MRPS35; MRPS28; HDCMD11P; MDS023; PSEC0213

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P82673

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-S35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRP-S35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP-S35 (I252) polyclonal antibody detects endogenous levels of MRP-S35 protein.

Bioworld Molecular Weight: ~ 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRP-S35 (I252) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate(40ug)
Lane 2: PC3 whole cell lysate(40ug)
Lane 3: A549 whole cell lysate(40ug)
Lane 4: K562 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human MRP-S35.

Conjugate: Unconjugated

Modification: Unmodified