MRP1 Polyclonal Antibody-BS90879

MRP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90879-50, BS90879-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P33527

Host: Rabbit

Reactivity: Human, Mouse, Rat,

Applications: WB, IHC, IF/ICC

All Applications: WB: 1:500~1:2000
IHC: 1:50~1:200
IF/ICC: 1:50~1:200

Background: The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an Mdr-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps expressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 protein is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues yet at much lower levels of expression.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: MRP1 polyclonal antibody detects endogenous levels of MRP1 protein.

Bioworld Molecular Weight: ~ 170-220 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using MRP1 antibody at 1:500 dilution., Western blot analysis of extracts of A-549 cells, using MRP1 antibody at 1:1000 dilution., Immunofluorescence analysis of HepG2 cells using MRP1 pAb at dilution of 1:50 (40x lens).

Alternative Name: ABCC1; ABC29; ABCC; GS-X; MRP; MRP1

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 210-304 of human MRP1/ABCC1 (NP_004987.2).

Conjugate: Unconjugated

Modification: Unmodified