Product Description

MRP2 Polyclonal Antibody

Catalogue Numbers: BS5801-50, BS5801-100

Sizes: 50µl, 100µl

Alternative Name: Canalicular multispecific organic anion transporter 1; ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2; ABCC2; CMOAT; CMOAT1; CMRP

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q92887

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter), and MRP3, which are both conjugate export pumps ex-pressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues, yet at much lower levels of expression.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP2 polyclonal antibody detects endogenous levels of MRP2 protein.

Bioworld Molecular Weight: ~ 195 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRP2 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse brain tissue lysate
Lane 3: H9C2 cell lyate

Immunogen: Synthetic peptide, corresponding to amino acids 1036-1081 of Human MRP2.

Conjugate: Unconjugated

Modification: Unmodified