Product Description

MRP3 (L1010) Polyclonal Antibody

Catalogue Numbers: BS3177-50, BS3177-100

Sizes: 50µl, 100µl

Alternative Name: Canalicular multispecific organic anion transporter 2; ATP-binding cassette sub-family C member 3; Multi-specific organic anion transporter D; MOAT-D; Multidrug resistance-associated protein 3; ABCC3; CMOAT2; MLP2; MRP3

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15438

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps ex- pressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also me- diates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP3 (L1010) polyclonal antibody detects endogenous levels of MRP3 protein.

Bioworld Molecular Weight: ~ 260  kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRP3 (L1010) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate(40ug)
Lane 2: Hela whole cell lysate(40ug)
Lane 3: PC12 whole cell lysate(40ug)
Lane 4: CT26 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 981-1030 of Human MRP3.

Conjugate: Unconjugated

Modification: Unmodified