MRPL13 Polyclonal Antibody-BS61532

MRPL13 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS61532-50, BS61532-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BYD1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB: 1:500~1:1000
IP: 1:50~1:200

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 96% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRPL13 polyclonal antibody detects endogenous levels of MRPL13 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of MRPL13 polyclonal antibody at 1:500 dilution
Lane 1: SK-OVCAR3 whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: AML-12 whole cell lysate (40ug)
Lane 5: PC12 whole cell lysate (40ug), Immunoprecipitation of HEK293T cell lysate using MRPL13 polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using MRPL13 #BS61532. “↑”（supernatant）;“↓(deposition）

Alternative Name: 39S ribosomal protein L13, mitochondrial; L13mt; MRP-L13

Immunogen: Synthetic peptide, corresponding to amino acids 125-169 of Human MRPL13.

Conjugate: Unconjugated

Modification: Unmodified