MRPL20 Polyclonal Antibody-BS65803

MRPL20 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65803-50, BS65803-100

Swiss-Prot: Q9BYC9

Host: Rabbit

MRPL20 Polyclonal Antibody-BS65803

Storage and Stability: Store at 4°C short term. Aliquot and store at -24°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. [provided by RefSeq, Jul 2008]

Purification and Purity: affinity purified by Protein A

Specificity: MRPL20 Polyclonal Antibody detects endogenous levels of MRPL20 protein.

Bioworld Molecular Weight: ~12 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse stomach)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRPL20) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (rat stomach)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRPL20) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: 39S ribosomal protein L20, mitochondrial; L20mt; MGC4779; RM20_HUMAN; MGC74465; Mitochondrial ribosomal protein L20; MRPL 20.

Immunogen: KLH conjugated synthetic peptide derived from human MRPL20:81-149/149

Modification: Unmodified

Conjugate: Unconjugated