Product Description

MRPL21 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65391-50, BS65391-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q7Z2W9

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Background: mitochondrial ribosomal protein L21(MRPL21) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Jul 2008],

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP-L21 Polyclonal Antibody detects endogenous levels of MRP-L21 protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using MRP-L21 Polyclonal Antibody diluted at 1：2000, Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MRPL21 Antibody. The picture on the right is blocked with the synthesized peptide., Western blot analysis of lysates from COLO cells, using MRPL21 Antibody. The Lane on the right is blocked with the synthesized peptide., Western blot analysis of the lysates from HeLa cells using MRPL21 antibody.

Alternative Name: MRPL21; 39S ribosomal protein L21; mitochondrial; L21mt; MRP-L21

Immunogen: The antiserum was produced against synthesized peptide derived from human MRPL21. AA range:131-180

Conjugate: Unconjugated

Modification: Unmodified