Product Description

MRPS35 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65234-50, BS65234-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: P82673

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Background: mitochondrial ribosomal protein S35(MRPS35) Homo sapiens Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MRP-S35 Polyclonal Antibody detects endogenous levels of MRP-S35 protein.

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of various cells using MRP-S35 Polyclonal Antibody, Western Blot analysis of K562 cells using MRP-S35 Polyclonal Antibody, Western blot analysis of lysates from Jurkat, COLO, and A549 cells, using MRPS35 Antibody. The Lane on the right is blocked with the synthesized peptide.

Alternative Name: MRPS35; MRPS28; HDCMD11P; MDS023; PSEC0213; 28S ribosomal protein S35; mitochondrial; MRP-S35; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt

Immunogen: The antiserum was produced against synthesized peptide derived from human MRPS35. AA range:211-260

Conjugate: Unconjugated

Modification: Unmodified