Product Description

MSH2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0152-50, MB0152-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P43246

Host: Mouse

Reactivity: Human

Applications: WB, ICC

All Applications: WB: 1:500~1000
ICC: 1:50~200

Background: Component of the post-replicative DNA mismatch repair system (MMR).Forms two different heterodimers:MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair.When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs.MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long.After mismatch binding,MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer,which is thought to be responsible for directing the downstream MMR events,including strand discrimination,excision,and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch:mismatched DNA provokes ADP-->ATP exchange,resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MSH2 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 100KDa
Observed band size: 100KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of MSH2 antibody in Hela, A431, A549 and 293T cell lysates using MSH2 antibody (1:500 diluted)., Immunocytochemistry staining of HeLa cells using anti-MSH2 antibody (dilution 1:100).

Alternative Name: DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2

Immunogen: Recombinant full length Human MSH2.

Conjugate: Unconjugated

Modification: Unmodified