Product Description

MSH6 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0153-50, MB0153-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P52701

Host: Mouse

Reactivity: Human

Applications: WB, ICC

All Applications: WB: 1:300~500
ICC: 1:50~100

Background: Component of the post-replicative DNA mismatch repair system (MMR).Heterodimerizes with MSH2 to form MutS alpha,which binds to DNA mismatches thereby initiating DNA repair.When bound,MutS alpha bends the DNA helix and shields approximately 20 base pairs,and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.After mismatch binding,forms a ternary complex with the MutL alpha heterodimer,which is thought to be responsible for directing the downstream MMR events,including strand discrimination,excision,and resynthesis.ATP binding and hydrolysis play a pivotal role in mismatch repair functions.The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange,resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.This transition is crucial for mismatch repair.MutS alpha may also play a role in DNA homologous recombination repair.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of MSH6 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size: 160KDa
Observed band size: 160KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot detection of MSH6 antibody in 293 and HCT116 cell lysates using MSH6 antibody (1:300-1:500 diluted)., Immunocytochemistry staining of HeLa cells using anti-MSH6 antibody (dilution 1:100).

Alternative Name: DNA mismatch repair protein Msh6; hMSH6; G/T mismatch-binding protein; GTBP; GTMBP; MutS-alpha 160 kDa subunit; p160; MSH6; GTBP

Immunogen: Recombinant full length Human MSH6.

Conjugate: Unconjugated

Modification: Unmodified